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KMID : 0861020130280030053
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Korea Journal of Herbology
2013 Volume.28 No. 3 p.53 ~ p.60
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김지은:Kim Ji-Eun
강옥화:Kang Ok-Hwa/김성배:Kim Sung-Bae/신인식:Shin In-Sik/강석훈:Kang Suk-Hoon/권동렬:Kwon Dong-Yeul/이승호:Lee Seung-Ho
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Abstract
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Objectives : DojukSan is known to be effective for treating a urinary diseases and stomatitis. However, there
has been a lack of studies regarding the effects of Dojuksan on the inflammatory activities and effector
inflammatory disease mechanism about macrophage before is not known. To elucidate the molecular mechanisms
of Dojuksan water extract (DJS) on pharmacological and biochemical actions in inflammation, we examined the
effect of DJS on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated macrophages.
Methods : In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked
immunosorbent assay, reverse transcription polymerase chain reaction, and western blot analysis to measure the
activation of MAPKs. Cells were treated with 200 ng/mL of LPS 1 h prior to the addition of DJS. Cell viability
was measured by MTS assay. The investigation focused on whether DJS inhibited nitric oxide (NO) and prostaglandin
E2 (PGE2) productions, as well as the expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2),
interleukin-6 (IL-6) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated RAW 264.7 cells.
Results : We found that DJS inhibited LPS-induced NO, PGE2 and IL-6 productions as well as the expressions
of iNOS and COX-2. Furthermore, DJS suppressed the LPS-induced phosphorylation of p38 MAPK and c-Jun
NH2-protein kinase (JNK).
Conclusions : These results suggest that DJS has inhibitory effects on LPS-induced PGE2, NO, and IL-6
production, as well as the expressions of iNOS and COX-2 in the murine macrophage. These inhibitory effects
occur through blockades on the MAPKs phosphorylation.
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KEYWORD
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Dojuksan (DJS), lipopolysaccharide (LPS), inflammation, RAW 264.7 Cells
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